蛋白定量 Protein Quantification (Bradford Assay)

蛋白定量 Protein Quantification (Bradford Assay)

Bradford法是一種快速，準確的方法來確定蛋白質的濃度。該法是基於這樣的觀察，Coomassie Brilliant Blue G-250變化從465納米到595納米的蛋白質結合時，酸性溶液中的最大吸光度發生。疏水和離子相互作用穩定的染料的陰離子形式，引起一個可見的顏色變化。的線性範圍內（5-25微克/毫升）的測定，目前更多的蛋白質，Coomassie Brilliant Blue結合。
Bradford assay is a rapid and accurate method to determine the concentration of protein. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mcg/ml), the more protein present, the more Coomassie binds.